Down-Regulation of Gephyrin and GABAA Receptor Subunits …
We examined for a correlation between total GABA A R subunit expression, plasma membrane expression and receptor composition. Analysis of tissue samples from the CA1 region of hippocampus show that SE promotes a loss of GABA A receptor subunits and of the scaffolding proteins associated with them.
Synaptic Gα12/13 signaling establishes hippocampal PV ... - PNAS
Impairment of the Gα12/13 pathway in postnatal hippocampal neurons diminishes inhibitory inputs without altering neuronal morphology or excitatory transmission. Gα12/13 signaling in hippocampal CA1 neurons in vivo selectively regulates PV interneuron synaptic connectivity, supporting an inhibitory synapse subtype-specific function of this pathway.
LPS induces microglial activation and GABAergic synaptic ... - Nature
2023年4月21日 · VGAT/gephyrin double-positive inhibitory puncta density was significantly reduced in all examined layers of hippocampal CA1 regions (SO, SP, SR and stratum lacunosum moleculare [SLM]) in LPS...
Gephyrin: a master regulator of neuronal function? - Nature
2014年2月20日 · a–c | Immunofluorescence staining reveals the expression of gephyrin (green) and the vesicular GABA transporter (VGAT; magenta), a marker of inhibitory presynaptic terminals, in the CA1...
Early alterations in hippocampal perisomatic GABAergic synapses …
2019年1月15日 · Quantification of overlapping PV/VGAT/gephyrin immunoreactivities in triple immunolabeled brain sections disclosed a statistically significant increase in the density of perisomatic GABAergic synapses originating from PV+ interneurons and terminating around pyramidal cells in both CA1 and CA3 subregions of the hippocampus of APPPS1 mice as ...
miRNA-mediated control of gephyrin synthesis drives sustained ...
2024年9月18日 · We identify miR153 as a novel regulator of Gphn mRNA translation which controls gephyrin protein levels and synaptic clustering, ultimately impacting inhibitory synaptic structure and function. iLTP induction downregulates miR153, reversing its translational suppression of Gphn mRNA and promoting de novo gephyrin protein synthesis and synaptic c...
Down‐regulation of gephyrin and GABA - Wiley Online Library
2013年1月7日 · The protein expression of gephyrin, GRIP, NSF, and GABARAP was analyzed in tissue samples from the CA1 region of hippocampus obtained at three time points after SE induction: 1 day (during the acute injury), 4 days (when interictal activity can be readily detected), and 8 days (when interictal activity becomes established and spontaneous ...
Structural Heterogeneity of the GABAergic Tripartite Synapse
2022年10月7日 · GABAergic synapses were labeled presynaptically using an antibody against the vesicular GABA transporter (VGAT), because VGAT localizes to synaptic vesicles of inhibitory terminals (GABAergic and glycinergic) and glycinergic synapses are believed to be absent or very rare in the CA1 stratum radiatum .
Gephyrin regulates GABAergic and glutamatergic synaptic
2011年6月10日 · Gephyrin is a scaffold protein essential for stabilizing glycine and GABA (A) receptors at inhibitory synapses. Here, recombinant intrabodies against gephyrin (scFv-gephyrin) were used to assess whether this protein exerts a transynaptic action on …
Synaptic recruitment of gephyrin regulates surface GABAA …
Immunostainings of intracellular proteins such as gephyrin (with mAb7a antibody) and vGAT were performed after fixation (PFA 4%), permeabilization (0.2% Triton X-100 for 10 min) and sequential incubation with primary and secondary antibodies.