Confocal Microscopy Reveals Cell Surface Receptor Aggregation …
This protocol quantitates the fluorescent intensity of labeled receptors as a function of the beam area of the confocal microscope. This provides a quantitative measure of the state of target molecule aggregation on the cell surface.
Use of Electron Microscopy in the Detection of Adrenergic Receptors
Adrenergic receptors (ARs) belong to a superfamily of the G-proteincoupled receptors and are categorized by their binding to endogenously occurring catecholamines, i.e., norepinephrine and epinephrine. Adrenergic receptors are classified into three groups (α 1 -, α 2, and β-ARs), each of which is further divided into three subtypes.
A live-imaging protocol for tracking receptor dynamics in single cells …
2022年6月17日 · Adjacent membrane receptors can show different cellular responses to ligand stimulation. Here, we describe a super-resolution microscopy imaging protocol for tracking the dynamics of two different membrane-bound receptors in single cells. We describe the transfection protocol by electroporation.
Live Cell Imaging Analysis of Receptor Function | SpringerLink
2009年10月24日 · Recent advances in microscopy techniques have made it possible to study receptor behaviour in live cells to gain a better understanding of dynamics, binding partners and sub-cellular localisation. Here we describe several common currently used techniques to study receptor behaviour in living cells.
Turn-key mapping of cell receptor force orientation and ... - Nature
2021年8月3日 · Building on this technology, we recently developed molecular force microscopy (MFM) which uses fluorescence polarization to map receptor force orientation with diffraction-limited resolution...
Super-resolution microscopy reveals unprecedented detail of …
2016年6月15日 · Salk scientists used light-sheet super-resolution imaging to capture the rearrangement of T-cell receptors from nanometer-scale protein islands (left) to micrometer-scale microclusters (right) after T-cell activation in mouse lymph nodes.
Physiology, Cellular Receptors - StatPearls - NCBI Bookshelf
2024年9月19日 · When a water-soluble ligand binds to a cell-surface receptor, it activates the receptor, initiating a signal transduction pathway that alters cellular function. The 3 types of cell-surface receptors include G protein–coupled receptors (GPCRs), ion channel receptors, and enzyme-linked receptors.
Turn-key mapping of cell receptor force orientation and …
2021年8月3日 · Building on this technology, we recently developed molecular force microscopy (MFM) which uses fluorescence polarization to map receptor force orientation with diffraction-limited resolution (~250 nm). Here, we show that structured illumination microscopy (SIM), a super-resolution technique, can be used to perform super-resolution MFM.
Subcellular and Synaptic Distribution of Muscarinic Receptors in ...
2024年7月13日 · Here, we describe the confocal immunofluorescence approach and high-resolution electron microscopic pre-embedding immunogold technique that we have developed to analyze the subcellular and synaptic distribution of two acetylcholine muscarinic receptors (MR), M 2 MR and M 4 MR in neurons in three different models including fixed brain sections, p...
Signalling under the microscope - Nature
2017年5月3日 · G-protein-coupled receptors are biological targets for drug discovery. Developments in cryo-electron microscopy have enabled the solution of the structure of a class B receptor in complex...