MES is used as a buffering agent in biology and biochemistry. It has pK a value of 6.15 at 20 °C. The pH (and pK a at ionic strength I≠0) of the buffer solution changes with concentration and temperature, and this effect may be predicted using online calculators. [2] MES is highly soluble in water. The melting point is approx. 300 °C.

MES (0.5 M, pH 6) preparation guide and recipe. Recipe can be automatically scaled by entering desired final volume. MES buffer is a Good's buffer that is remarkably stable both chemically and enzymatically. It has minimal UV absorbance and is often used …

By using MES as an experimental medium or additive, the response and tolerance of biological systems to drugs or potential toxic substances can be evaluated. Product packaging. These widespread applications demonstrate the diversity of MES as a buffer, as well as its importance and practicality in the laboratory.

With excellent water solubility and virtual insolubility in lipids, MES Buffer is impermeable to membranes, ensuring efficacy in cellular environments. Its applications span maintaining stability in cell culture media, protein-based buffer formulations, and electrophoresis running buffers.

Aug 1, 2015 · The best way of making MES buffer buffer is to start with a solution of MES free acid at required molarity and adjust the pH with NaOH. The sodium salt is made internally and the molarity...

MES Buffer stands out as an optimal choice for capillary electrochromatography, offering low ionic mobility. Notably, it serves as a safer alternative to potentially toxic agents like cacodylate and non-zwitterionic buffers, including citrate and malate.

Order high-quality MES hydrate buffer from Sigma-Aldrich. Ideal for applications in Molecular Biology, Cell Biology and Biochemical research within the pH range of 5.5-6.7.

Dec 27, 2024 · The Ultimate Guide to 0.1mol/L MES Buffer: Uses, Properties, and Safety Guidelines. 2-(N-Morpholine)ethanesulfonic acid, commonly abbreviated as MES, is a widely used biological buffer known for its exceptional buffering capabilities in the pH range of 5.5 to 6.7.

MES (2-Morpholinoethanesulphonic acid) is a buffering agent in biology and biochemistry. MES is one of the Good's buffers, the buffer capacity ranging pH 5.5-7.0. MES is broadly used to regulate pH value for plants culture medium, reagent solution, and physiological experiments.

The buffering range of MES buffer is pH 5.5-6.7. If the experiment needs to maintain a stable pH environment within this pH range, MES buffer is a good choice. At the same time, the buffering capacity of the buffer should also be considered, that is, the ability of the buffer to resist external acid-base influences. 2.