Choose a buffer based on your pH requirements as well as the pKa, a measure of acid strength that accounts for pH, concentration, and temperature. Regulatory or purity needs for your exact application should also be considered. The following tables can help you navigate preparation of many common buffer solutions by pH and pKa.

The following table applies to RICCA CHEMICAL COMPANY reference standard buffers only. Buffer formulations with the same pH but different ingredients or concentrations may have different temperature dependence profiles. The pH at 25°C for our reference standard buffer line is traceable to NIST standard

The charts below can help you find the ideal buffer to establish and maintain optimal pH by taking into account your type of organism, the qualities of different buffer types, the impact of pKa (concentration and temperature), and the complexation of …

You can find the composition of NEB buffers and diluents in the table below. The columns represent the diluent or buffer referenced, and the rows represent the individual diluent or buffer components. The squares within the table indicate the final concentration of components for a 1X concentration of the buffer or diluent defined by the column.

Buffer Table Crystal Growth 101 page 2 TRIS hydrochloride pKa 25°C 8.1 TAPSO pKa 25°C 7.6 BICINE pKa 25°C 8.3 Tricine pKa 25°C 8.1 HEPBS pKa 25°C 8.3 TAPS pKa 25°C 8.4 AMPD pKa 25°C 8.8 Glycine pKa 25°C (1) 2.3 (2) 9.6 CAPSO pKa 25°C 9.6 TABS pKa 25°C 8.9

Presented are tables to assist with the selection and preparation of commonly used buffers for use in biotechnology laboratories. The control and maintenance of pH in biological experiments is achieved by selection of a suitable buffering agent.

Choose a buffer based on your pH requirements as well as the pKa, a measure of acid strength that accounts for pH, concentration, and temperature. Regulatory or purity needs for your exact application should also be considered. The following tables can help you navigate preparation of many common buffer solutions by pH and pKa. Buffer Level.

RICCA CHEMICAL COMPANY offers a complete line of pH buffers ranging from pH 1 to pH 13. Commonly used buffers (pH 4, 7 and 10) are available as clear or color-coded reference standard solutions for easier identification in the lab. Alternate color-coding is available for other pH values.

Orion™ pH Buffer Part No. 910168 9116860 910104 910425 910410-WA 910460 9104CB 700001 700402 910105 910686 916860 700001 700702 910107 910710 910725 ... Low Ionic Strength Buffer Low Ionic Strength Buffer Color-coded Yellow Low Ionic Strength Buffer Color-coded Blue °C 1.678 4.010 4.10 5.00 6.865 6.97 7.00 9.15 9.184 10.013 12.460 0 1.667 4. ...

Thermo Scientific pH meters contain pH versus temperature values for commonly used buffers. This allows the meter to recognize a particular pH buffer and calibrate with the correct buffer value at the measured temperature. measurements is temperature. Temperature. Isopotential Point 0 …