Automated Sequencing - an overview | ScienceDirect Topics
Automated DNA Sequencing. Automated sequencing has been developed to sequence a really large amount of DNA. This procedure uses the principle of the Sanger chain-termination method.
Automated DNA Sequencing - Magazine Science
2016年11月26日 · Automated DNA sequencing involves four fluorophores, one for each of the four nucleotide bases. The resulting fluorescent signal is recorded at a fixed point when DNA passes through a capillary containing an electrophoretic gel.
Overview of Next Generation Sequencing Technologies - PMC
High throughput DNA sequencing methodology (next generation sequencing; NGS) has rapidly evolved over the past 15 years and new methods are continually being commercialized. ... We prefer to use the term second generation, third generation, etc. realizing that the automated AB sequencing machine technology described above is really the ...
Sequencing Genomes - Genomes - NCBI Bookshelf
Techniques for sequencing DNA are clearly of central importance in this context and we will begin the chapter with a detailed examination of sequencing methodology.
Digital-WGS: Automated, highly efficient whole-genome sequencing …
2020年12月9日 · Here, we describe Digital-WGS, a sample preparation platform that streamlines high-performance single-cell WGS with automatic processing based on digital microfluidics. Using the method, we provide high single-cell capture efficiency for any amount and types of cells by a wetted hydrodynamic structure.
A primer on current and common sequencing technologies
2013年7月18日 · DNA sequencing with a Nanopore instrument relies on converting of electrical signal of nucleotides by passing through a nanopore which is an ±-hemolysin pore covalently attached with a cyclodextrin molecule (i.e., the binding site for nucleotides) embedded in reconstituted lipid bilayers.
DNA Sequencing Technologies | Learn Science at Scitable - Nature
In 1986, a company named Applied Biosystems began to manufacture automated DNA sequencing machines based on the Sanger method (Figure 1). These machines used fluorescent dyes to tag each...
Next-generation DNA sequencing | Nature Biotechnology
2008年10月9日 · Next-generation DNA sequencing has the potential to dramatically accelerate biological and biomedical research, by enabling the comprehensive analysis of genomes,...
Large-Scale and Automated DNA Sequence Determination
1991年10月4日 · DNA sequence analysis is a multistage process that includes the preparation of DNA, its fragmentation and base analysis, and the interpretation of the resulting sequence information.
Automated DNA sequencing | Nucleic Acids Sequencing: A …
DNA sequencing has undergone great advances since the initial developments of Maxam and Gilbert (1) and Sanger et al (2) in the 1970s. The Maxam“Gilbert or chemical method first employs specific chemicals to modify DNA at one or two bases. The DNA is then cleaved at the modified site to generate fragments shorter by one base than the targeted base.