The RecE recombination pathway mediates recombination …
1990年7月1日 · Escherichia coli generalized recombination, utilizing the RecA RecB recombination pathway, requires large stretches (70–200 bp) of complete DNA sequence homology. In contrast, we have found that the RecE pathway can promote recombination between DNA with only short stretches of homology.
The RecE Pathway of Bacterial Recombination | SpringerLink
There appear to be three pathways of recombination active or potentially active in strains of Escherichia coli (Clark, 1971, 1973). The operation of two of these three pathways has so far been associated with nuclease activities. The RecBC pathway makes use of...
RecE/RecT and Redα/Redβ initiate double-stranded break repair …
First, RecE or Redα degrades the DNA in a 5′–3′ direction, starting from the DSB, thereby creating a 3′ ssDNA overhang. Then, RecT or Redβ binds to the ssDNA, forming a recombinogenic proteonucleic filament which is used in recombination, either by …
Homologous pairing proteins encoded by the Escherichia coli recE …
Early genetic analysis of alternate recombination pathways in Escherichia coli identified the RecE recombination pathway and the required exonuclease VIII encoded by the recE gene. Observations that not all recombination events promoted by the RecE pathway require recA suggest the existence of an ad …
Roles of RecJ, RecO, and RecR in RecET-Mediated Illegitimate ...
We analyzed effects of overexpression of RecE and RecT on illegitimate recombination during prophage induction in Escherichia coli and found that frequencies of spontaneous and UV-induced illegitimate recombination are enhanced by coexpression of RecE and RecT in the wild type, but the enhanced recombination was reduced by recJ, recO, or recR ...
Genetic analysis of the RecE pathway of genetic recombination …
The RecE pathway of genetic recombination in Escherichia coli K-12 was defined to be the pathway that is utilized in deoxyribonucleic acid exonuclease V (ExoV)-defective cells which express constitutively recE+, the structural gene for deoxyribonucleic acid exonuclease VIII.
The RecE PATHWAY OF BACTERIAL RECOMBINATION Jane R. Gillen and Alvin J. Clark Departmen& of Molecular Biology, University of California, Berkeley, California 94720 There appear to be three pathways of recombination active or potentially active in strains of Escherichia coli (Clark, 1971, 1973).
Involvement of RecE exonuclease and RecT annealing protein …
1994年1月28日 · We demonstrated that a double-stranded (ds) gap in DNA is repaired by a gene conversion mechanism in an Escherichia coli recBC sbcA23 strain, as predicted by the ds break repair models for homologous recombination. The sbc A mutation is known to induce several gene products encoded on the Rac prophage present in most strains of E. coli K-12.
mologous recombination in bacteria was associated with the sexual process of conjugation and was viewed as an evolutionary mechanism both for shuffling the genome and for spreading favorable alleles. However, more recently, a more immediate function of homol- ogous recombination has been recognized: namely, it
Full-length RecE enhances linear-linear homologous recombination …
2012年4月29日 · In this work, we discovered that full-length RecE along with RecT efficiently facilitates homologous recombination between two linear molecules. We used this pair of proteins to clone large...