The protein was renatured by removing SDS with two washes of 20 mM sodium acetate buffer, pH 5.0, and incubation in 50 mL of 20 mM sodium acetate buffer with 2.5% Triton X-100 (v/v) for 3 h, with ...

citric acid/sodium acetate (pH 3.0-5.0), sodium phosphate (pH 6.0-7.5), Tris-HCl (pH 7.0-9.0), glycine-NaOH (pH 9.0-10.0) and phosphate-NaOH (pH 11.0). For the pH stability studies, the purified ...

Department of Biochemistry and Biophysics, Texas A&M University, 301 Old Main Drive, College Station, Texas 77845, United States ...

The cytoplasmic domain of Sso1 and truncation constructs were dialyzed against 20 mM sodium phosphate buffer, pH 6.5, containing 50 mM NaCl. Protein concentrations were determined by internally ...

2011). Protein pellets recovered from culture supernatant and containing moss-produced rhEPO were dissolved in a 100 mM sodium acetate buffer containing 2% SDS (pH 4.0). After 10 min shaking (1,200 ...

SH-SY5Y CTSD KO and H4 CTSD KO cells overexpressing NCL10/AD- and PD-associated CTSD variants were lysed in a Triton-based buffer (50 mM sodium acetate, 0.1 M NaCl, 1 mM EDTA, 0.2% Triton X-100, pH ...

Protein Purification. Frozen cells were thawed and resuspended in lysis buffer (50 mM Tris, pH 7.5/400 mM NaCl/20 mM imidazole/5 mM 2-mercaptoethanol/5% glycerol/1% Triton X-100/one tablet of protease ...