The protein was renatured by removing SDS with two washes of 20 mM sodium acetate buffer, pH 5.0, and incubation in 50 mL of 20 mM sodium acetate buffer with 2.5% Triton X-100 (v/v) for 3 h, with ...

The cytoplasmic domain of Sso1 and truncation constructs were dialyzed against 20 mM sodium phosphate buffer, pH 6.5, containing 50 mM NaCl. Protein concentrations were determined by internally ...

citric acid/sodium acetate (pH 3.0-5.0), sodium phosphate (pH 6.0-7.5), Tris-HCl (pH 7.0-9.0), glycine-NaOH (pH 9.0-10.0) and phosphate-NaOH (pH 11.0). For the pH stability studies, the purified ...

Center of Nuclear Energy in Agriculture, University of São Paulo, Av. Centenário 303, 13400-970 Piracicaba, SP, Brazil Institute of Science and Technology, Sao Paulo State University, Av. Três de ...

Department of Biochemistry and Biophysics, Texas A&M University, 301 Old Main Drive, College Station, Texas 77845, United States ...

Department of Microbiology, Kakatiya University, Warangal, India. Synthetic dyes are extensively used in the textile industries and significant proportion appears in the form of wastewater and is ...

The type VI secretion system (T6SS) is a sophisticated, contact-dependent nanomachine involved in interbacterial competition. To function effectively, the T6SS must penetrate the membranes of both ...

Chatsworth, CA). In the second workflow (B), 500 µl of MacKenzie buffer, i.e. [4M guanidine thiocyanate, 0.2 M sodium acetate (pH 5.0), 25 mM ethylenediaminetetraacetic acid (EDTA), 2.5% (w/v) ...

In an additional group of rats, a polyethylene catheter (PE-10 soldered to a PE-50) was inserted into the abdominal aorta through the femoral artery with rats under ether anesthesia. Twenty-four hours ...