Here we explore peptide inhibitors of Cdc20, a substrate-recognition subunit and activator of the E3 ubiquitin ligase the anaphase promoting complex/cyclosome (APC/C) that is essential in mitosis and ...

Here, we use a synthetic amphiphile to modulate the membrane mechanical properties of cell-derived vesicles and probe channel activation. Using precise membrane mechanical characterization approaches ...

Phosphate and MES buffers were prepared (0.1 M and 5 mM ... DARCO ® 100 mesh particle size activated charcoal (Millipore Sigma; Burlington, MA) and vortexed briefly. After vortexing, the samples were ...

Medium pH was adjusted to 6.2 with NaOH 40%. Further reactor experiments with moderate-strength buffered media (25 mM) were conducted using the same medium composition except for the buffers, which ...